Human mitochondria (the energy-producing organelles outside the nucleus of cells) DNA is passed down generations only through females. Thus, testing of the DNA in mitochondria can aid in establishing matrilineal lines. (Establishing matrilineal lines with documents is more difficult than for patrilineal lines in most European lines because the surnames of females are lost in naming children; exceptions are Spanish and Portuguese.)
mtDNA is a circular structure composed of 16,569 nucleotides, also called base pairs. (It is believed that the mitochondria were originally parasitic bacteria which, through evolution, became symbiotic with the human cell and then became an integral part of the cell; bacterium DNA is circular.) There is a 400 base-pair section labeled 16001-16400 that evolves faster than other sections, so it is used to distinguish human groups that evolved separately. In the table below there are two sets of four rows that list the 400 base pairs: the first row starts at position 16001 and ends at 16100, the second row encompasses 16101-16200, the third row encompasses 16201-16300 and the fourth row encompasses 16301-16400. The Cambridge Reference Sequence (CRS) is the sequence of base pairs for an arbitrary person to which all other persons are compared.
Average number of mtDNA base pairs differences between two humans is about 10-66. The HVR1 test indicates to which of the major 36 World (10 for 99% of Europeans) haplogroups you belong. This could be millions of people! If it is a rare haplogroup, it could be a much smaller group. Mine is haplogroup I (Iris), in which are <2% of Europeans. This is most helpful for genealogy when you think you are related to someone through your maternal line. Test HVR1 for both to see if they are the same; if the same there is a 50% probability that you have a common ancestor 52 generations ago or less. For better resolution, do the HVR2 test; if still the same there is a 50% probability that you have a common ancestor 28 generations ago or less. So, it is not accurate enough for genealogy.
Full sequencing of mtDNA is equivalent to 41 markers on the Y-chromosome for genealogical use in the strict female line. It is difficult to follow a maternal line back many generations, since surnames usually change each generation.
I have determined the mtDNA pattern for my daughters and their matrilineal line by having one of my granddaughters provide the cheek cells. Their earliest known maternal ancestor is Caroline Theiss. Their result is given in the table below in the second set of four rows of base pairs with the differences from the CRS in bold red. (There are four different base pairs labeled by Adenine, Cytosine, Guanine and Thymine.)
mtDNA HRV1 results for the
daughters and granddaughters of L. David Roper. (Starts at 16001, ends at 16569
= 569 base pairs.) (Donor: Eva Marie Oliver, granddaughter of L. David Roper) |
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Cambridge Reference
Sequence: Eva Marie Oliver relative mutations with CRS (16069T & 16126C): |
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16001-10 | 16011-20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | Add |
ATTCTAATTT | AAACTATTCT | CTGTTCTTTC | ATGGGGAAGC | AGATTTGGGT | ACCACCCAAG | TATTGACTCA TATTGACTTA |
CCCATCAACA | ACCGCTATGT | ATTTCGTACA | +0 |
TTACTGCCAG | CCACCATGAA | TATTGTACGG TATTGCACGG |
TACCATAAAT | ACTTGACCAC | CTGTAGTACA | TAAAAACCCA | ATCCACATCA | AAACCCCCTC | CCCATGCTTA | +100 |
CAAGCAAGTA | CAGCAATCAA | CCCTCAACTA | TCACACATCA | ACTGCAACTC | CAAAGCCACC | CCTCACCCAC | TAGGATACCA | ACAAACCTAC | CCACCCTTAA | +200 |
CAGTACATAG | TACATAAAGC | CATTTACCGT | ACATAGCACA | TTACAGTCAA | ATCCCTTCTC | GTCCCCATGG | ATGACCCCCC | TCAGATAGGG | GTCCCTTGAC | +300 |
CACCATCCTC | CGTGAAATCA | ATATCCCGCA | CAAGAGTGCT | ACTCTCCTCG | CTCCGGGCCC | ATAACACTTG | GGGGTAGCTA | AAGTGAACTG | TATCCGACAT | +400 |
CTGGTTCCTA | CTTCAGGGTC | ATAAAGCCTA | AATAGCCCAC | ACGTTCCCCT | TAAATAAGAC | ATCACGATG | +500 |
Age ranges for some founding haplogroups (http://www.stats.ox.ac.uk/~macaulay/papers/richards_2000.pdf) (http://www.hotep.bigstep.com/27082001.htm). |
Note that, in this study, haplogroup J, where Eva's mtDNA lies, is 6.1% of the European population in this study and originated c10,000 ybp. |
Graph taken from http://www.chitpavans.com/chitpavangenetics2.html Note that the origin of haplogroup J (Jasmine) is Central and West Eurasia. |
Migration map for founding haplogroups, courtesy of
Family Tree DNA Note that haplogroup ... originated in ... area. |
The major haplogroups for Europeans are H, J, U, T, K, X, V and I. (See Bryan Syke's book The Seven Daughters of Eve.)
haplogroup | % of Europeans | years before present | origin |
H (Helena) | 47 | 20,000 | South France |
J (Jasmine) | 17 | 10,000 | Middle East |
U (Ursula) | 11 | 45,000 | Greece |
T (Tara) | 9 | 17,000 | Tuscany |
K (Katrina) | 6 | 15,000 | North Italy |
X (Xenia) | 6 | 25,000 | Georgia, Asia |
V (Velda) | 5 | 17,000 | North Spain |
I (Iris) | 2 | 26,000 | ? |
The major haplogroups for Europeans are H, J, U, T, K, X, V and I. (See Bryan Syke's book The Seven Daughters of Eve)
View all known female descendants (and their husbands) of Caroline Theiss.
Roper
Genetics Project
Franklin Genetics
Project
Little/Klein/Cline Genetics
Project